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Image Search Results
Journal: International journal of molecular sciences
Article Title: Identification of Moesin (MSN) as a Potential Therapeutic Target for Colorectal Cancer via the β-Catenin-RUNX2 Axis.
doi: 10.3390/ijms241310951
Figure Lengend Snippet: Figure 7. MSN upregulated RUNX2 expression in CRC cells. Expression levels of MSN and RUNX2 were determined through RT-qPCR. (A) Silencing of MSN reduced the expression of RUNX2 in HCT 116 cells. (B) The nuclear translocation of β-catenin was determined through Western blotting of cytoplasmic and nuclear extracts. PARP and GAPDH were used as loading controls for nuclear and cytoplasmic fractions, respectively. (C) Levels of Phospho-GSK3β-S9 and total GSK3β were determined in vector and MSN over DLD-1 cells through Western blotting. GAPDH is shown as a loading control. RUNX2 and MMP9 were evaluated through RT-qPCR in the presence of β-catenin inhibitors, ICG-001 and PKF118-310, in HCT 116 cells (D) and vector and MSN-overexpressing DLD-1 cells (E). RUNX2 expression was determined through RT-qPCR in scrambled control and MSN-KD HCT 116 cells after treatment with a GSK3β inhibitor, TWS 119 treatment (F). * p < 0.05, ** p < 0.01.
Article Snippet: The membranes were probed using the following primary antibodies at 4 ◦C overnight:
Techniques: Expressing, Quantitative RT-PCR, Translocation Assay, Western Blot, Plasmid Preparation, Control